Cellulase biosynthesis in a catabolite repression-resistant mutant of Thermomonospora curvata.
نویسندگان
چکیده
A catabolite repression-resistant mutant of the thermophilic actinomycete Thermomonospora curvata was obtained by treatment with ethyl methanesulfonate and UV light. Cellulase biosynthesis was undiminished by glucose, 2-deoxyglucose, or alpha-methyl glucoside, which are potent repressors in the wild type. Intracellular cyclic AMP levels were higher in the mutant in both the absence and the presence of repressors.
منابع مشابه
Cellulase production by Thermomonospora curvata isolated from municipal solid waste compost.
A cellulolytic, thermophilic actinomycete (previously isolated from municipal refuse compost samples) was identified as Thermomonospora curvata. A determination was made of the optimal conditions for cellulase production by T. curvata when grown at 55 C in a medium containing mineral salts, cellulose, and yeast extract. The pH and temperature optima (pH 6.0 and 65 C) for the cellulase produced ...
متن کاملAmylase production by thermomonospora curvata.
Thermomonospora curvata produces an extracellular inducible amylase which does not accumulate products repressive to cellulase production during growth on starch-cellulose ratios similar to those of compost.
متن کاملCellulolytic activity of Thermomonospora curvata: optimal assay conditions, partial purification, and product of the cellulase.
Thermomonospora curvata produces cellulases active against both cotton fibers (designated C(1) activity) and carboxymethylcellulose (C(x) activity). In reaction systems employing optimal substrate concentration, pH, and temperature, hydrolysis rates (measured by the release of soluble reducing sugars) were initially linear and decreased on prolonged incubation, although only a small amount of s...
متن کاملNucleo-cytoplasmic shuttling dynamics of the transcriptional regulators XYR1 and CRE1 under conditions of cellulase and xylanase gene expression in Trichoderma reesei
Trichoderma reesei is a model for investigating the regulation of (hemi-)cellulase gene expression. Cellulases are formed adaptively, and the transcriptional activator XYR1 and the carbon catabolite repressor CRE1 are main regulators of their expression. We quantified the nucleo-cytoplasmic shuttling dynamics of GFP-fusion proteins of both transcription factors under cellulase and xylanase indu...
متن کاملA deduced Thermomonospora curvata protein containing serine/threonine protein kinase and WD-repeat domains.
The gene pkwA coding for a typical WD-repeat protein was found in the chromosome of the bacterium Thermomonospora curvata CCM 3352. Until now WD-repeat proteins were through to be confined to eukaryotes.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Applied and environmental microbiology
دوره 47 1 شماره
صفحات -
تاریخ انتشار 1984